CMOS Nanoelectrode Array for All-Electrical Intracellular Electrophysiological Imaging

February 21, 2017

Figure 3: Network-level intracellular recording of in vitro neonatal rat ventricular cardiomyocyte cultures*.
[Reprinted by permission from Nature Publishing Group: Nature Nanotechnology ©2017]

In a new Nature Nanotechnology article, Hongkun Park and Donhee Ham (SEAS) groups describe a new tool they developed which is capable of high-precision electrophysiological recording of a large network of electrogenic cells - a long outstanding challenge in neurobiology and cardiology. The scientists combined nanoscale intracellular electrodes with complementary metal-oxide-semiconductor (CMOS) integrated circuits to realize a high-fidelity all-electrical electrophysiological imager for parallel intracellular recording at the network level. Their CMOS nanoelectrode array has 1,024 recording/stimulation 'pixels' equipped with vertical nanoelectrodes, and can simultaneously record intracellular membrane potentials from hundreds of connected in vitro neonatal rat ventricular cardiomyocytes. The authors demonstrate that this network-level intracellular recording capability can be used to examine the effect of pharmaceuticals on the delicate dynamics of a cardiomyocyte network, thus opening up new opportunities in tissue-based pharmacological screening for cardiac and neuronal diseases as well as fundamental studies of electrogenic cells and their networks.

*See Jeffrey Abbott, Tianyang Ye, Ling Qin, Marsela Jorgolli, Rona S. Gertner, Donhee Ham & Hongkun Park, "CMOS nanoelectrode array for all-electrical intracellular electrophysiological imaging," Nature Nanotechnology (2017) doi:10.1038/nnano.2017.3.